Colorimetric Micromethod for Protein Determination with Erythrosin B

✍ Scribed by H.S. Soedjak

• Book ID: 102965137
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 547 KB
• Volume: 220
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1310

✦ Synopsis

Reaction of erythrosin B with proteins results in a stable, highly colored chromophore with an absorbance maximum at (545 \mathrm{~nm}). This is the basis for a new quantitative determination method of proteins in solution. The assay can be performed at room temperature, but is faster and more sensitive at (90-95^{\circ} \mathrm{C}). The erythrosin assay is characterized by (i) stable dye-protein color, (ii) high sensitivity ((2-14 \mu \mathrm{g} / \mathrm{ml}) protein), (iii) short reaction time (1.5-2 (\min) at (90-95^{\circ} \mathrm{C}) ), (iv) good reproducibility, (v) limited interference by common reagents, and (vi) low protein-to-protein variability. Thus, the erythrosin assay can be useful for routine analytical purposes and may overcome some of the limitations of other currently employed assays. c 1994 Academic Press, Inc.