Microdetermination of monosaccharides in glycoproteins by gas-liquid chromatography

✍ Scribed by Milan Tomana; William Niedermeier; Cooper Spivey

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 584 KB
• Volume: 89
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90731-5

✦ Synopsis

In a newly developed method for determining picomolar quantities of monosaccharides in glycoproteins, the methyl glycosides released by methanolysis of glycoproteins are separated as trifluoroacetates by gas-liquid chromatography and are detected with an electron capture detector. The application of the method has been demonstrated for the analysis of the carbohydrate moiety of secretory component isolated from human colostral immunoglobulin A.