Microbial aspartase and its activity on deamination of L-aspartyl-L-phenylalanine methyl ester
โ Scribed by Wonyoon Chung; Yang Mo Goo
- Book ID
- 112915162
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 433 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0253-6269
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๐ SIMILAR VOLUMES
## Abstract The stability of USAL (ฮฑโLโaspartylโLโphenylalanine methyl ester hydrochloride) was studied in solutions in relation to the pH value (1.10โ6.95 at 20 ยฐC), the temperature (20โ80 ยฐC at pH 2.2) and the ionic strength (0.16โ2.0 at 20 ยฐC and pH 4.0). The effects of pH and temperature of the
Aspartyl-L-phenylalanine methyl and ethyl esters were synthesised enzymatically in toluene by means of the zinc protease thermolysin adsorbed onto Celite R-640 ยฎ , which is a porous support able to control the hydration of the protein. The conversion of the two derivatised amino acids into the desir