High isolated yields in thermolysin-catalysed synthesis of Z-l-aspartyl-l-phenylalanine methyl ester in toluene at controlled water activity
✍ Scribed by Luigi De Martin; Cynthia Ebert; Lucia Gardossi; Paolo Linda
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- French
- Weight
- 57 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0040-4039
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✦ Synopsis
Aspartyl-L-phenylalanine methyl and ethyl esters were synthesised enzymatically in toluene by means of the zinc protease thermolysin adsorbed onto Celite R-640 ® , which is a porous support able to control the hydration of the protein. The conversion of the two derivatised amino acids into the desired products was complete, leading to >90% isolated yields. Moreover, working with equimolar concentrations of the reactants no purification steps were required. Thermolysin adsorbed onto Celite R-640 ® is shown to be a practical tool to synthesise biologically active peptides in organic media.