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Micro high-performance liquid chromatographic separations of bile acids

✍ Scribed by Toyohide Takeuchi; Shinji Saito; Daido Ishii

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
632 KB
Volume
258
Category
Article
ISSN
1873-3778

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✦ Synopsis

Separations of bile acids by micro high-performance liquid chromatography (micro-HPLC) using an immobilized 3a-hydroxysteroid dehydrogenase post-column and a spectrofluorimeter are described. The sensitivity of detection is greatly increased by pre-mixing /?-nicotinamide adenine dinucleotide with the mobile phase, using a single pump. The detection limit is 0.040.7 ng, depending on the solute. Instruments suitable for the analysis of bile acids in micro-HPLC are discussed.

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