Bile acids: LXXII. High-performance liquid chromatographic analysis of bile acid coenzyme A derivatives

✍ Scribed by David A. Abbott; David E. Schlarman; Ping H. Patrick; Daniel M. Tal; William H. Elliott

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 415 KB
• Volume: 146
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90566-4

✦ Synopsis

The mobilities of coenzyme A and coenzyme A derivatives of cholate, chenodeoxycholate, deoxycholate, lithocholate, and their 501 analogs were studied in reversed-phase high-performance liquid chromatography. With a C,s Radial-PAK A cartridge (lo-pm particles) and a solvent mixture of 2-propanol/lO mM phosphate buffer (pH 7.0, 140:360), separation of the chenodeoxycholyl and deoxycholyl coenzyme A derivatives was not observed. An increase in ionic strength of the buffer to 50 mM afforded separation, which was markedly augmented with a Cls Radial-PAK A cartridge with 5-pm particles. Lowering the pH of the buffer to 5.5 did not materially change the separations regardless of the ionic strength. Quantitation was carried out to a lower level of 8.5 X IO-'* mol. 0 1985 Academic press, hc.