Methods for fractionation and scintillation counting of radioisotope-labeled polyacrylamide gels
β Scribed by Samuel Ward; David L. Wilson; James J. Gilliam
- Book ID
- 107711090
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 977 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0003-2697
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Large (200 mm3) slices of polyacrylamide gels crosslinked with N,N'-diallyltartardiamide which contain tritium-labeled protein are readily solubilized in periodic acid for liquid scintillation counting of radioactivity, but the apparent recovery of label never exceeds 82%. Extraction of the slices w
A method is described for analyzing the radioactivity of 3H-labeled RNA after separation by gel electrophoresis. The gels were soaked in 10% acetic acid and were sliced. The gel slices were dehydrated in alcohol, then saturated with toluene, and finally permeated with a toluene-based scintillation f