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Methods for enzymatic and colorimetric determinations of d-erythrulose (d-tetrulose)

โœ Scribed by Kiyoka Morii; Saburo Hosomi; Tomoyuki Terada; Tadashi Mizoguchi

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
309 KB
Volume
151
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

The specific determinations of 6erythrulose by enzymatic assay or calorimetric method, which permit the quantitative determination of between 20 and 400 nmol of the sugar, are described. Enzymatic determination of Derythrulose made use of the D-erythrulose reductase purified from beef or chicken liver, which catalyzes specifically the reduction of D-erythrulose with concomitant conversion of NADH to NAD+. The calorimetric microdetermination of erythrulose could be carried out by utilizing the phenol-sulfuric acid reaction under low temperature. These methods are simple, rapid, and sensitive, and give reproducible results. o 1985 Academic press. IIK.

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