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Metachromatic assay for the quantitative determination of bacterial endotoxins

✍ Scribed by Tibor Keler; Alois Nowotny

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
406 KB
Volume
156
Category
Article
ISSN
0003-2697

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✦ Synopsis

A metachromatic

dye. 1.9-dimethylmethylene blue (DMB) reacts with bacterial endotoxins. This interaction results in a shift of the absorption maximum of DMB to shorter wavelengths. The findings indicate that the negatively charged lipid moiety of the endotoxic lipopolysaccharide reacts with DMB. The lowest amount of endotoxin detectable by the procedure described here is approximately one microgram.

The dye could not be used m the presence of serum components. DMB mixed to column chromatographic effluent showed good resolution in continuous monitoring of endotoxin components leaving the column.

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