Metabolic studies of mammalian cells by 31P-NMR using a continuous perfusion technique
โ Scribed by Knop, Richard H.; Chen, Chi-Wan; Mitchell, James B.; Russo, Angelo; McPherson, Scott; Cohen, Jack S.
- Book ID
- 122996232
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 833 KB
- Volume
- 804
- Category
- Article
- ISSN
- 0167-4889
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๐ SIMILAR VOLUMES
A perfusion technique applicable to metabolic studies by NMR is described in detail. The utility and versatility of the approach are demonstrated by following gluconeogenesis from [2-'3C]pyruvate in the pehsed, isolated mouse liver, and lipogenesis from [2-I3C]acetate in perfused Acantharnoeba cast
We describe an improved perfusion system designed for NMR studies with 13C-labeled metabolites Using this system we have monitored the uptake and metabolism of [ I3C]acetate and ['3Crplucose in pooled human lymphocytes cas1 in an agar-gel thread. o 1986 Academic Rm Inc