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A versatile perfusion technique for metabolic studies by NMR

✍ Scribed by Yves Geoffrion; Sylvain Lareau; Roxanne Deslauriers; Keith Butler; Michael Pass; Ian C. P. Smith

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 470 KB
Volume: 2
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910020108

No coin nor oath required. For personal study only.

✦ Synopsis

A perfusion technique applicable to metabolic studies by NMR is described in detail.

The utility and versatility of the approach are demonstrated by following gluconeogenesis from [2-'3C]pyruvate in the pehsed, isolated mouse liver, and lipogenesis from [2-I3C]acetate in perfused Acantharnoeba castellanii cells embedded in agarose filaments.

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