A perfusion technique applicable to metabolic studies by NMR is described in detail. The utility and versatility of the approach are demonstrated by following gluconeogenesis from [2-'3C]pyruvate in the pehsed, isolated mouse liver, and lipogenesis from [2-I3C]acetate in perfused Acantharnoeba cast
A double-tuned probe for metabolic NMR studies
β Scribed by Elhadi Najim; Jean-Philippe Grivet
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 272 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0740-3194
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β¦ Synopsis
Abstract
A doubleβtuned single coil probe for high field metabolic NMR studies has been designed and tested. The resonant coil is a singleβturn of copper foil wound on a quartz cylinder. It is fed by a short length of parallel strip line made of the same material; the feeder is also used as a tuning capacitance. Electrical parameters (quality factors, efficiencies at proton and carbon frequencies) and magnetic resonance data (90Β° pulse lengths, minimum decoupler power) for lossy samples have been measured. The probe allows for the optimal use of the limited high homogeneity region of a vertical magnet. It shows good sensitivity and efficient decoupling. Β© 1992 Academic Press, Inc.
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