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A perfusion technique for 13C NMR studies of the metabolism of 13C-labeled substrates by mammalian cells

✍ Scribed by Robbe C. Lyon; Patrick J. Faustino; Jack S. Cohen

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 508 KB
Volume: 3
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910030503

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✦ Synopsis

We describe an improved perfusion system designed for NMR studies with 13C-labeled metabolites Using this system we have monitored the uptake and metabolism of [ I3C]acetate and ['3Crplucose in pooled human lymphocytes cas1 in an agar-gel thread. o 1986 Academic Rm Inc

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