A13C NMR study of the application of [U-13C] succinate for metabolic investigations in rabbit renal proximal convoluted tubular cells

✍ Scribed by Arnold W. H. Jans; Rudolph Willem

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 362 KB
• Volume: 14
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910140114

✦ Synopsis

Abstract

The fate of the ^13^C label arising from [ U‐^13^C ] succinate in rabbit renal proximal convoluted tubular (PCT) cells, incubated with [U‐^13^C]succinate, was investigated by ^13^C NMR spectroscopy of the perchloric acid extracts of the cell pellets. The ^13^C label of [U‐^13^C] succinate was predominantly metabolized into glutamate, glutamine, and into glucose, lactate, and alanine, which is the consequence of a carbon efflux of the label from the Krebs cycle at the stage of α‐ketoglutarate and oxaloacetate, respectively. © 1990 Academic Press, Inc.