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Metabolic fate of L-[methyl-11C]methionine in human plasma

✍ Scribed by Kiichi Ishiwata; Jun Hatazawa; Kazuo Kubota; Motonobu Kameyama; Masatoshi Itoh; Taiju Matsuzawa; Toshihiro Takahashi; Ren Iwata; Tatsuo Ido

Book ID
104682673
Publisher
Springer
Year
1989
Tongue
English
Weight
492 KB
Volume
15
Category
Article
ISSN
0340-6997

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Measurement of l-[methyl-11C]methionine
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The routine measurement of L-[methyl-11C]methionine in human plasma after i.v. injection of the tracer is described. The protein-free plasma metabolites of the tracer were analyzed using a cation-exchange column (Aminex A-6) eluted with a pH 4.25 citrate buffer. The ratios of L-[methyl-11C]methionin

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Carbon 11 which is a 20.4 minutes half life isotope emitting positrons was used for methionine labelling on the methyl group by action of 11C-methyl-iodide on DL or L homocysteine. With the method described, 20 to 30 mCi of 11C-methyl-methionine (Specific activity 50 mCi/muM) may be obtained within

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On-line ["Clmethylation using ["Clmethyl iodide has been successfully applied to the preparation of L-[methyl-"Clmethionine ("C-Met). ["C]Methyl iodide is first trapped in a short column that contains Porapak Q and L-homocysteine thiolactone coated on inert support. The column is then charged with N