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Mechanisms of the irreversible inactivation of horseradish peroxidase caused by hydroxymethylhydroperoxide

✍ Scribed by Stefan Marklund


Book ID
115701625
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
945 KB
Volume
154
Category
Article
ISSN
0003-9861

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Horseradish peroxidase which had been aminated by periodate oxidation and reductive amination was purified by cation-exchange chromatography on S-Sepharose. Instead of the expected single peak of aminated enzyme, two distinct peaks of protein were eluted from the column. Evaluation of the protein in