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Mechanism of chromosome banding: A phenomenological approach

โœ Scribed by Manjubala S. Talekar; Subhash V. Talekar


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
661 KB
Volume
20
Category
Article
ISSN
0020-7608

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โœฆ Synopsis


Abstract

A quantitative expression is derived, using Fick's diffusion law, for varieties of experimentally observed band patterns in metaphase chromosomes.


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Mechanisms of chromosome banding
โœ David E. Comings ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Springer ๐ŸŒ English โš– 731 KB

The interaction of Hoechst 33258 with DNA has been examined to help clarify the mechanisms of banding. 1. In agreement with previous studies Hoechst fluorescence is enhanced to a greater degree in AT-rich compared to GC-rich DNA. 2. Hoechst causes an increase in the DNA Tm which is greater at the hi

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The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was trea

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โœ Tadashi A. Okada; David E. Comings ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› Springer ๐ŸŒ English โš– 933 KB
Mechanisms of chromosome banding
โœ David E. Comings; Maximo E. Drets ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Springer ๐ŸŒ English โš– 779 KB

Prior studies on subfractions of mouse and Kangaroo rat DNA have suggested that variations in base concentration within a given genome may not be great enough to account for Q-banding. To examine this with another species, calf DNA was subfractionated by CsCl ultracentrifugation into GC-rich satelli

Molecular basis of chromosome banding
โœ K. Simola; R.-K. Selander; A. Chapelle ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Springer ๐ŸŒ English โš– 325 KB

Silver and mercury ions are known to react with the bases of nucleic acids in solution. At low cation/base ratios Ag+ has an affinity for GC pairs in DNA, whereas Hg++ is preferentially bound to AT-rich nucleic acids. We have used fluorometry to measure the effect of these cations on the fluorescenc