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Studies on the mechanism of chromosome banding with trypsin

โœ Scribed by C. Lundsteen; Lene Kristoffersen; F. Ottosen


Publisher
Springer
Year
1974
Tongue
English
Weight
168 KB
Volume
24
Category
Article
ISSN
0340-6717

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The role of trypsin in the pre-treatment
โœ Bruce R. Korf; Barbara E. Schuh; Martin J. Salwen; Dorothy Warburton; Orlando J. ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Springer ๐ŸŒ English โš– 434 KB

The role of trypsin in the elicitation of G-banding on human chromosomes was studied in two separate laboratories. Enzyme activity and ability of trypsin to chelate calcium were manipulated by dilution of the treatment solution, and by inhibition with diisopropylphosphofluoridate, diphenylcarbamyl c