Mechanisms of chromosome banding

✍ David E. Comings 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 731 KB

The interaction of Hoechst 33258 with DNA has been examined to help clarify the mechanisms of banding. 1. In agreement with previous studies Hoechst fluorescence is enhanced to a greater degree in AT-rich compared to GC-rich DNA. 2. Hoechst causes an increase in the DNA Tm which is greater at the hi

✍ David E. Comings; Evangelita Avelino 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 892 KB

The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was trea

Mechanisms of chromosome banding

✍ David E. Comings; Maximo E. Drets 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 779 KB

Prior studies on subfractions of mouse and Kangaroo rat DNA have suggested that variations in base concentration within a given genome may not be great enough to account for Q-banding. To examine this with another species, calf DNA was subfractionated by CsCl ultracentrifugation into GC-rich satelli

Mechanisms of Giemsa banding

✍ H. E. Wyandt; Ruth S. Anderson; S. R. Patil; F. Hecht 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 416 KB

Molecular basis of chromosome banding

✍ K. Simola; R.-K. Selander; A. Chapelle; G. Corneo; E. Ginelli 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 447 KB

The effects of mouse satellite, main band and total DNA on the fluorescence intensity of quinacrine and of the bibenzimidazole derivative Hoechst 33258 were tested in solution. No significant differences were noticed between the double-stranded DNAs in spite of the 5% difference in AT-content betwee

Molecular basis of chromosome banding

✍ K. Simola; R.-K. Selander; A. Chapelle 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 325 KB

Silver and mercury ions are known to react with the bases of nucleic acids in solution. At low cation/base ratios Ag+ has an affinity for GC pairs in DNA, whereas Hg++ is preferentially bound to AT-rich nucleic acids. We have used fluorometry to measure the effect of these cations on the fluorescenc