Mechanism of activity-linked ionization in carbonic anhydrase
โ Scribed by Raj K. Gupta; John M. Pesando
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 266 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0020-7608
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Cell extracts (27000 x g supernatant) of acetate grown Methanosarcina barkeri were found to have carbonic anhydrase activity (0.41 U/mg protein), which was lost upon heating or incubation with proteinase K. The activity was inhibited by Diamox (apparent Ki = 0.5 mM), by azide (apparent Ki = I raM),
N4-(5-Nitro-2-pyridyl)-4-homosulfanilamide-A mixture of 2chloro-5-nitropyridine (0.25 g.), 4-homosulfanilamide hydrochloride (0.32 g.), and freshly fused sodium acetate (0.2 8.) was refluxed with 30 ml. absolute ethanol for 24 hr., after which the reaction mixture was diluted with water until comple
Carbonic anhydrase (CA, EC 4.2.1.1) activity was detected in 22 species of tropical cnidarians which contain endosymbiotic dinoflagellates (= zooxanthellae). CA activity was 2 to 3 times higher in animal tissue than in algae and ca, 29 times higher in zooxanthellate than azooxanthellate species. It