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Measurement of testosterone with a high-performance liquid chromatograph equipped with a flow-through ultraviolet spectrophotometer

โœ Scribed by Roger C. Cochran; Kirwin J. Darney Jr.; Larry L. Ewing

Book ID
104144008
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
487 KB
Volume
173
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

A technique is described for measuring nanogram amounts of testosterone using high-performance liquid chromatography with detection by a fiow-through spectrophotometer. The addition of a non-radioisotopic internal standard @androsten-I I&&3, ! ?-&one) to the biological specimen automaticahy . . corrects for testosterone losses due to extraction and non-quantitative sample injection into the high-performance liquid chromatograph. This new method, which can be performed by inexperienced personnel, is shown to be rapid, precise; accurate and specific for testosterone.

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