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Measurement of protein using flow injection analysis with bicinchoninic acid

✍ Scribed by Lawrence C. Davis; Gary A. Radke

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 382 KB
Volume: 161
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90665-8

No coin nor oath required. For personal study only.

✦ Synopsis

We have used the bicinchoninic acid reagent developed by Pierce Chemical Co. to measure proteins in a simple flow injection analyzer. The sensitivity is comparable to that of the Lowry method and no pipetting of reagents is needed. Results are obtained in less than 1 min and samples may be run at a rate of 60/h. The response is linear over a range of protein concentration (O-IO rg) and sample size (5-20 ~1) convenient for most analytical requirements. A peristaltic pump, a controlled-temperature water bath, and a spectrophotometer with flow cuvette are the only special apparatus required. 0 1987 Academic press. IX.

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