We have used the bicinchoninic acid reagent developed by Pierce Chemical Co. to measure proteins in a simple flow injection analyzer. The sensitivity is comparable to that of the Lowry method and no pipetting of reagents is needed. Results are obtained in less than 1 min and samples may be run at a
Protein measurement using bicinchoninic acid: elimination of interfering substances
β Scribed by Rhoderick E. Brown; Kari L. Jarvis; Kristi J. Hyland
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 394 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0003-2697
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The bicinchoninic acid (BCA) assay method for the determination of protein has been investigated for its utility in measuring the protein content of plasma lipoproteins. Although other methods, principally those based on the method of Lowry et al. (1951, J. Biol. Chem. 193, 265-275) have been extens
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