Measurement of plasma acetate kinetics using high-performance liquid chromatography

✍ Scribed by M. Persson; B. Bleiberg; D. Kiss; J. Miles

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 553 KB
• Volume: 198
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(91)90520-4

✦ Synopsis

Previous studies suggest that plasma acetate may be an important fuel in man, accounting for approximately 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of D-beta-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [14C]acetate into red blood cells was negligible.