𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Measurement of intracellular pH using flow cytometry with carboxy-SNARF-1

✍ Scribed by Eric D. Wieder; Haiying Hang; Michael H. Fox

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
475 KB
Volume
14
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Use of flow cytometry and SNARF to calib
Use of flow cytometry and SNARF to calibrate and measure intracellular pH in NS0 cells
✍ Jennifer Bond; Julie Varley πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 153 KB

## Abstract ## Background Two calibration methods have been proposed for determining the relation between the fluorescence ratio of a pH‐sensitive fluorescent indicator and intracellular pH (pH~i~). The first method uses nigericin to clamp pH~i~ to external pH (pH~e~) and the second is the null po

Detection of apoptosis by flow cytometry
Detection of apoptosis by flow cytometry of cells simultaneously stained for intracellular pH (carboxy SNARF-1) and membrane permeability (Hoechst 33342)
✍ Jason E. Reynolds; Jinfang Li; Alan Eastman πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 789 KB

Intracellular acidification appears to be a frequent and possibly universal occurrence during apoptosis. We have used carboxy SNARF-1 to measure intracellular pH by flow cytometry. We report here that the addition of Hoechst 33342 concurrently with carboxy SNARF-1 provides a clearer &ctiminatlon of

Simultaneous measurement of Ca2+ and pH
Simultaneous measurement of Ca2+ and pH by laser cytometry using fluo-3 and SNARF-1
✍ Toshiko Atsumi; Kenji Sugita; Minoru Kohno; Tsutomu Takahashi; Takao Ueha πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 921 KB

We present a new conwnient method for simultaneous measurement of intracellular calcium concentration ([Caz+]3 and inlracellular pH (pH3 using laser cytometry with a mixture of fho-3 (for [Ca2']3 and SNARF-1 (for pH3, with iso excitation (488 -)-dual emission (530 nm for fluo-3 and > 630 n m for SNA