Intracellular pH measurements using flow cytometry with 1,4-diacetoxy-2,3-dicyanobenzene
โ Scribed by Dr. John A. Cook; Michael H. Fox
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 660 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We present a new conwnient method for simultaneous measurement of intracellular calcium concentration ([Caz+]3 and inlracellular pH (pH3 using laser cytometry with a mixture of fho-3 (for [Ca2']3 and SNARF-1 (for pH3, with iso excitation (488 -)-dual emission (530 nm for fluo-3 and > 630 n m for SNA
A sensitive HPLC-fluorescence method for determining total endogenous plasma homocysteine (Hcy), cysteine (Cys) and cysteinylglycine (Cys-Gly) following derivatization with ammonium 7-fluoro 2,1,3-benzoxadiazole-4-sulphonate (SBD-F) is described. Quantitation utilizes an internal standard, 2-mercapt