✦ LIBER ✦

Ratiometric measurement of intracellular pH in cultured human keratinocytes using carboxy-SNARF-1 and flow cytometry

✍ Scribed by Piet E. J. Van Erp; Monique J. J. M. Jansen; Gijs J. De Jongh; Jan B. M. Boezeman; Joost Schalkwijk

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 563 KB
Volume: 12
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.990120205

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Measurement of intracellular pH using fl

Measurement of intracellular pH using flow cytometry with carboxy-SNARF-1

✍ Eric D. Wieder; Haiying Hang; Michael H. Fox 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 475 KB

Use of flow cytometry and SNARF to calib

Use of flow cytometry and SNARF to calibrate and measure intracellular pH in NS0 cells

✍ Jennifer Bond; Julie Varley 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB

## Abstract ## Background Two calibration methods have been proposed for determining the relation between the fluorescence ratio of a pH‐sensitive fluorescent indicator and intracellular pH (pH~i~). The first method uses nigericin to clamp pH~i~ to external pH (pH~e~) and the second is the null po

Detection of apoptosis by flow cytometry

Detection of apoptosis by flow cytometry of cells simultaneously stained for intracellular pH (carboxy SNARF-1) and membrane permeability (Hoechst 33342)

✍ Jason E. Reynolds; Jinfang Li; Alan Eastman 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 789 KB

Intracellular acidification appears to be a frequent and possibly universal occurrence during apoptosis. We have used carboxy SNARF-1 to measure intracellular pH by flow cytometry. We report here that the addition of Hoechst 33342 concurrently with carboxy SNARF-1 provides a clearer &ctiminatlon of