Measurement of hydrogen exchange rates using 2D NMR spectroscopy
โ Scribed by Christopher M Dobson; Lu-Yun Lian; Christina Redfield; Karen D Topping
- Publisher
- Elsevier Science
- Year
- 1986
- Weight
- 585 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0022-2364
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๐ SIMILAR VOLUMES
A procedure to measure exchange rates of fast exchanging protein amide hydrogens by time-resolved NMR spectroscopy following in situ initiation of the reaction by diluting a native protein solution into an exchanging deuterated buffer is described. The method has been used to measure exchange rates
Translational diffusion measurements are a useful tool for where g is the gyromagnetic ratio, d the gradient duration, studying supramolecular complexes and for characterizing the as-G the gradient strength, and D the interval between PFG sociation state of molecules that aggregate at NMR concentrat