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Measurement of four-bond HN-HαJ-couplings in staphylococcal nuclease

✍ Scribed by Geerten W. Vuister; Ad Bax

Book ID
104652176
Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
512 KB
Volume
4
Category
Article
ISSN
0925-2738

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✦ Synopsis

Quantitative J-correlation and triple-resonance ECOSY-type experiments are used to unambiguously establish the presence of four-bond sequential HN-H ~ J-couplings in the protein staphylococcal nuclease.

Substantially negative 4JHc~HN values, ranging from -0.8 to -2.3 Hz, are observed when the ~ angle is near + 120 ~ and the following d? angle near +60 ~ For other conformations, the four-bond HN-H ~ J-couplings fall between -0.5 and +0.5 Hz.

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