Accurate Measurement of HN–Hα Residual Dipolar Couplings in Proteins

✍ Scribed by Mengli Cai; Hong Wang; Edward T. Olejniczak; Robert P. Meadows; Angelo H. Gunasekera; Nan Xu; Stephen W. Fesik

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 61 KB
• Volume: 139
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1819

✦ Synopsis

A method for accurately measuring H(N)-H(alpha) residual dipolar couplings is described. Using this technique, both the sign and magnitude of the coupling can be determined easily. Residual dipolar coupling between H(N)(i)-H(alpha)(i) and H(N)(i)-H(alpha)(i-1) were measured for the FK506 binding protein complexed to FK506. The experimental values were in excellent agreement with predictions based on an X-ray crystal structure of the protein/ligand complex, suggesting that these residual dipolar couplings will provide accurate structural constraints for the refinement of protein structures determined by NMR.