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Measurement of amino acid compositions of glycoprotein systems by gas-phase hydrolsis and reversed-phase high-performance liquid chromatography

✍ Scribed by Deborah E.H. Palladino; Robin M. House; Kenneth A. Cohen


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
848 KB
Volume
599
Category
Article
ISSN
1873-3778

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All 24 dansyl amino acids were separated by reverse-phase high-performance liquid chromatography on Develosil C8-5, using a linear gradient made from Tris-HCl buffer (pH 7.75) and methanol. A linear relationship between the amount of sample and peak area was found over the range of 6 to 300 ng (0.02