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Measurement of 2-deoxyglucose and 2-deoxyglucose 6-phosphate in tissues

✍ Scribed by Jill K. Manchester; Maggie M.-Y. Chi; Joyce G. Carter; Mary Ellen Pusateri; David B. McDougal; Oliver H. Lowry

Book ID: 102985091
Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 836 KB
Volume: 185
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90265-b

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✦ Synopsis

The enzymatic methods previously described for 2-deoxyglucose (DG) and 2-deoxyglucose 6-phosphate have been refined and adapted to measurements of brain samples ranging from 50 mg wet weight to less than a microgram dry weight. Procedures for preparing such samples for assay are described. Analytical properties of the enzymes employed are given together with means for overcoming their possible short comings. Emphasis is placed on information useful for employing DG to assess rapid changes in glucose metabolism.

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