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Enzymatic assays for 2-deoxyglucose and 2-deoxyglucose 6-phosphate

โœ Scribed by Maggie M.-Y. Chi; Mary Ellen Pusateri; Joyce G. Carter; Beverly J. Norris; David B. McDougal Jr.; Oliver H. Lowry

Book ID
102629335
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
468 KB
Volume
161
Category
Article
ISSN
0003-2697

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The enzymatic methods previously described for 2-deoxyglucose (DG) and 2-deoxyglucose 6-phosphate have been refined and adapted to measurements of brain samples ranging from 50 mg wet weight to less than a microgram dry weight. Procedures for preparing such samples for assay are described. Analytica

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Akabayashi et al. made a valuable modification of the enzymatic methods from our laboratory for measuring 2-deoxyglucose and 2-deoxyglucose 6-phosphate. Their modified procedure eliminates glucose and glucose 6-phosphate by conversion to fructose-1,6-bisphosphate, thereby saving two analytical steps