Dephosphorylation of 2-deoxyglucose 6-phosphate and 2-deoxyglucose export from cultured astrocytes
โ Scribed by R.J. Forsyth; K. Bartlett; J. Eyre
- Book ID
- 116113753
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 687 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0197-0186
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๐ SIMILAR VOLUMES
The enzymatic methods previously described for 2-deoxyglucose (DG) and 2-deoxyglucose 6-phosphate have been refined and adapted to measurements of brain samples ranging from 50 mg wet weight to less than a microgram dry weight. Procedures for preparing such samples for assay are described. Analytica
Akabayashi et al. made a valuable modification of the enzymatic methods from our laboratory for measuring 2-deoxyglucose and 2-deoxyglucose 6-phosphate. Their modified procedure eliminates glucose and glucose 6-phosphate by conversion to fructose-1,6-bisphosphate, thereby saving two analytical steps