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Mass spectrometry of high-mannose oligosaccharides after trifluoroacetolysis and periodate oxidation

✍ Scribed by Peter Lipniunas; Anne-Sophie Angel; Karin Erlansson; Frank Lindh; Bo Nilsson

Book ID
102988804
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
866 KB
Volume
200
Category
Article
ISSN
0003-2697

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✦ Synopsis

High-mannose oligosaccharides were treated with a mixture of trifluoroacetic acid and trifluoroacetic anhydride under conditions where reducing terminal N-acetylglucosamine residues are specifically degraded. The resulting mannose-containing products were, after further chemical modifications, analyzed by mass spectrometry in fast atom bombardment and electron ionization modes. Binding positions between monosaccharide residues were deduced from mass spectra of peracetylated compounds, which, prior to the derivatization, had been subjected to periodate oxidation and borodeuteride reduction.

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