Structural Analysis of O-Linked Oligosaccharide-Alditols by Electrospray–Tandem Mass Spectrometry after Mild Periodate Oxidation and Derivatization with 2-Aminopyridine
✍ Scribed by Willy Morelle; Jérôme Lemoine; Gérard Strecker
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 208 KB
- Volume
- 259
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
O-linked oligosaccharide-alditols were analyzed by a combination of high-performance liquid chromatography (HPLC) and electrospray-tandem mass spectrometry (ESI-MS/MS). First, oligosaccharide-alditols were treated with sodium meta-periodate under conditions where core N-acetylgalactosaminitol is specifically degraded. The resulting fragments were labeled with 2-aminopyridine and purified on a reversed-phase column. Pyridylamino oligosaccharides yielded protonated molecular ions in positive-ion ES-MS and gave Y-series sequence ions, arising from glycosidic cleavages, by ESI-tandem mass spectrometry. Information on sugar sequence and branching of oligosaccharides linked at C6 and C3 to the N-acetylgalactosaminitol can be obtained. A systematic study of various oligosaccharide-alditols demonstrated that this approach constitutes a powerful tool for the structural characterization of O-glycans available only in limited quantities.