Mass Spectrometry in the Biological Sciences

✍ Scribed by Judith L. Vaitukaitis, Maureen Mylander (auth.), A. L. Burlingame, Steven A. Carr (eds.)

Publisher: Humana Press
Year: 1996
Tongue: English
Leaves: 568
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Leading practitioners describe in detail advanced methods of mass spectrometry used in structural characterization of biomacromolecules of both natural and recombinant origin. They demonstrate by example how these methodologies can solve a wide array of real-world problems in protein biochemistry, immunology, and glycobiology, as well as for human bacterial pathogens, lipids, and nucleic acids. The book offers a unique opportunity to learn these techniques that are revolutionizing the field. Its authoritative assessment in the context of how to solve important and challenging problems in bioscience and medicine ensures a competitive advantage for today's researchers.

✦ Table of Contents

Front Matter....Pages i-xii
Mass Spectrometry in the Health and Life Sciences....Pages 1-4
Designing Time-of-Flight Mass Spectrometers for the Future: Multichannel Recording of Peptide Amino Acid Sequences....Pages 5-23
The Role of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in Biological Research — New Developments and Applications....Pages 25-68
Electrospray Ionization Mass Spectrometry: The Observation of Covalent, Ionic and Non-Covalent Interactions.....Pages 69-84
Using Deuterium Exchange and Mass Spectrometry to Study Protein Structure....Pages 85-104
MALDI-TOF Mass Spectrometry in the Protein Biochemistry Lab: From Characterization of Cell Cycle Regulators to the Quest for Novel Antibiotics....Pages 105-133
Chemistry, Mass Spectrometry and Peptide-Mass Databases: Evolution of Methods for the Rapid Identification and Mapping of Cellular Proteins....Pages 135-150
Identification of Electroblotted Proteins by Peptide Mass Searching of a Sequence Database....Pages 151-170
Mass Spectrometric Identification of Proteins Isolated By Two-Dimensional Gel Electrophoresis....Pages 171-202
Infrared-Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (IR-MALDI-MS) of Proteins Electroblotted onto Polymer Membranes After SDS-PAGE Separation....Pages 203-216
Picoliter Vials: A New Sample Handling Technique for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry....Pages 217-226
Direct Neuropeptide Profiling of Single Neurons and Target Tissue by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry....Pages 227-243
Approaches to the Practical Use of MS/MS in a Protein Sequencing Facility....Pages 245-265
Capillary Liquid Chromatography/Electrospray Mass Spectrometry with a Magnetic Sector Instrument for Accurate Mass Measurement of Peptide and Protein Mixtures....Pages 267-280
Mass Spectrometric Methods for Peptide Sequencing: Applications to Immunology and Protein Acylation....Pages 281-305
A Multifaceted Strategy for the Characterization of Recombinant gD-2, a Potential Herpes Vaccine....Pages 307-331
Identifying Unexpected Protein Modifications....Pages 333-350
Mass Spectrometry in Carbohydrate Recognition Studies....Pages 351-364
Mass Spectrometric and Tandem Mass Spectrometric Approaches to the Analysis of Glycoconjugates....Pages 365-384
Structural Determination and Biosynthetic Studies of the Rhizobial Nod Metabolites: The Lipo-Chitin Oligosaccharides....Pages 385-401
Mass Spectrometry as a Tool to Determine the Structures and Heterogeneity of Bacterial Lipooligosaccharides (LOS)....Pages 403-424
Applications of Tandem Liquid Chromatography/Mass Spectrometry in Drug Biotransformation and Quantification Studies....Pages 425-449
Isoeicosanoids: Mass Spectrometry of Free Radical Oxidation Products Derived From Arachidonoyl Phospholipids....Pages 451-476
Applications of Accelerator Mass Spectrometry in Toxicology: A Highly Sensitive Tool for Low-Level Isotope Measurements....Pages 477-495
Characterization of Posttranscriptional Modification in Nucleic Acids by Tandem Mass Spectrometry....Pages 497-517
The Future of DNA Sequencing: Methods and Applications....Pages 519-533
Back Matter....Pages 535-570

✦ Subjects

Analytical Chemistry

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