✍ Scribed by A. L. Burlingame (Eds.)
No coin nor oath required. For personal study only.
This volume describes and integrates the techniques and fundamentals of more than a decade of revolutionary advances in both chromatographic and mass spectrometric technologies that have enabled the direct investigation of biomacromolecules per se and have provided the analytical power base to usher in the new fields of proteomics and systems biology.
It also covers new biophysical applications such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. Finally it describes atto-to-zepto-mole quantitation of 14C and 3H by accelerator mass spectrometry.
Part 1 of 2 volumes about Mass Spectrometry
Authoritative and comprehensive treatment of protein mass spectrometry in human cell biology
Presents fundamentals, techniques, instrumentation and bioinformatics
Provides an overview of proteomics, protein-protein and protein-ligand binding, and biophysical studies
Content:
Mass Spectrometers for the Analysis of Biomolecules Review Article
Pages 3-48
Michael A. Baldwin
Hybrid Quadrupole/Time‐of‐Flight Mass Spectrometers for Analysis of Biomolecules Review Article
Pages 49-78
Werner Ens, Kenneth G. Standing
Tandem Time‐of‐Flight Mass Spectrometry Review Article
Pages 79-108
Marvin L. Vestal, Jennifer M. Campbell
Tandem Mass Spectrometry in Quadrupole Ion Trap and Ion Cyclotron Resonance Mass Spectrometers Review Article
Pages 109-148
Anne H. Payne, Gary L. Glish
Collision‐Induced Dissociation (CID) of Peptides and Proteins Review Article
Pages 148-185
J. Mitchell Wells, Scott A. McLuckey
Peptide Sequencing by MALDI 193‐nm Photodissociation TOF MS Review Article
Pages 186-209
Joseph W. Morgan, Justin M. Hettick, David H. Russell
Peptide Sequence Analysis Review Article
Pages 209-244
Katalin F. Medzihradszky
Proteomics Review Article
Pages 245-289
John T. Stults, David Arnott
Bioinformatic Methods to Exploit Mass Spectrometric Data for Proteomic Applications Review Article
Pages 289-312
Robert J. Chalkley, Kirk C. Hansen, Michael A. Baldwin
Protein Conformations, Interactions, and H/D Exchange Review Article
Pages 312-360
Claudia S. Maier, Max L. Deinzer
Ligand–Metal Ion Binding to Proteins: Investigation by ESI Mass Spectrometry Review Article
Pages 361-389
Noelle Potier, HГ©lГЁne Rogniaux, Guillaume Chevreux, Alain Van Dorsselaer
Site‐Specific Hydrogen Exchange of Proteins: Insights into the Structures of Amyloidogenic Intermediates Review Article
Pages 389-402
Zhong‐ping Yao, Paula Tito, Carol V. Robinson
Quantitating Isotopic Molecular Labels with Accelerator Mass Spectrometry Review Article
Pages 402-422
John S. Vogel, Adam H. Love
Accelerator Mass Spectrometry for Biomedical Research Review Article
Pages 423-443
Karen Brown, Karen H. Dingley, Kenneth W. Turteltaub
Series Editors
Page ii
Contributors to Volume 402
Pages vii-viii
Preface
Pages ix-x
Methods in Enzymology
Pages xi-xxxiii
Author Index
Pages 445-470
Subject Index
Pages 471-478
Table of Contents
Pages v-vi
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