Mass culture of mammalian cells as a national project
β Scribed by Isao Yamane
- Book ID
- 104814891
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 162 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0920-9069
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Nonwoven fabrics were used as a support matrix for culturing anchorage-dependent diploid human lung fibroblast (IMR-90) cells. The mast significant advantage of the fabrics is that low-inocula concentrations suffice to attain high final cell density. Cultures were successfully grown from inocula con
A n c h o r a g e -d e p e n d e n t m a m m a l i a n cells were cultivated at high cell density in a novel culture system using p o l y u r e t h a n e f o a m ( P U F ) as a s u b s t r a t u m for cell attachment. P U F has a m a c r o p o r o u s structure giving a high surface a r e a to v o l
## Abstract Chinese hamster cells (line CHO) were cloned in suspension with a carboxymethylcelluloseβcontaining medium, and the colonies were counted with an electronic particle counter after enzymatically degrading the carboxymethylcellulose with cellulase. Survival of xβirradiated cells was deter