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Marine Corrinoid-Binding Proteins for the Direct Determination of Vitamin B12 by Radioassay

✍ Scribed by Mukesh K. Sahni; Stamatios Spanos; Miryam Z. Wahrman; Gurdial M. Sharma

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
111 KB
Volume
289
Category
Article
ISSN
0003-2697

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✦ Synopsis

Under conditions of vitamin B12 deficiency the marine phytoplankton Thalassiosira pseudonana secretes into the growth medium a protein (Gm protein) that binds B12 specifically with an affinity constant of 2 x 10(11) M(-1). When Gm protein was used as a B12-specific binder in radioassays for the direct determination of the vitamin, the detection limit of the technique approached 5 pg B12/mL. In its natural state, Gm protein is oligomeric having molecular weight of over 400 kDa. Modification of the solution environment of this protein produces unique changes in its tertiary and quaternary structure. The amino acid and submit composition of the protein is reported.

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