Localization of the gene for the vitamin B12 binding protein, transcobalamin II, near the centromere on mouse chromosome 11, linked with the hemoglobin alpha-chain locus
✍ Scribed by M. Fràter-Schröder; M. Prochazka; O. Haller; F. Arwert; H. J. Porck; L. C. Skow; L. -G. Lundin; J. Hilkens; J. Hilgers
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 684 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-2928
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✦ Synopsis
Somatic cell hybrids', recombinant inbred (RI) mouse strains, and backcross breeding experiments were used to locate the gene of transcobalamin H (Tcn-2), the vitamin BI 2 binding protein in mouse serum. TCN-2 was found to be a useful genetic marker in the somatic cell hybrids. Selected hybrid clones were derived from fusions between GR mouse cells and the Chinese hamster cell line E36. Analysis of mouse specific chromosomal enzyme markers in relationship to TCN-2 secretion, in the hybrid clones, provided provisional evidence for assignment of the Tcn-2 locus to chromosome I1. The strain distribution pattern of the TCN-2 variants S and F in the RI series CXS, constructed from the cross of BALB/cHeA (TCN-2S) with STS/A (TCN-2F), implied a close linkage with the hemoglobin alpha-chain locus (Hba) on chromosome 1 I. Backcross breeding using inbred strains confirmed