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Marcophate colony-stimulating factor in nerve growth factor preparations

✍ Scribed by C. L. Erickson-Miller; C. N. Abboud; R. W. Stach; C. N. Frantz; J. K. Brennan

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
509 KB
Volume
19
Category
Article
ISSN
0360-4012

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✦ Synopsis

Following a report that nerve growth factor preparations have granulocyte-colony-stimulating activity, we investigated the presence of colony-stimulating factors in 7s mouse submaxillary nerve growth factor and its subunits. Macrophage colonies were formed in mouse bone marrow cultures after exposure to preparations of 7s nerve growth factor, the y subunit, and, to a small extent, the a subunit; the p subunit, which is responsible for the nerve growth function, did not stimulate colony growth. Furthermore, the esteropeptidase activity of the y subunit was not detected in preparations of macrophage colony-stimulating factor purified from the giant cell tumor (GCT) cell line. Immunoprecipitation of radiolabeled y subunit with a polyclonal antibody to L-cell macrophage colonystimulating factor showed a protein band that could represent the y subunit of nerve growth factor. Separation of the macrophage activity from the esteropeptidase activity of the y subunit was accomplished on the basis of molecular size. Thus, macrophage colonystimulating factor was a contaminant of nerve growth factor produced by the mouse submaxillary gland and copurified with the y subunit.

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