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Mapping of the gene specifying DNA polymerase III of Bacillus subtilis

✍ Scribed by Love, Evelyn ;D'Ambrosio, Joseph ;Brown, Neal C. ;Dubnau, David

Publisher: Springer
Year: 1976
Tongue: English
Weight: 641 KB
Volume: 144
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00341730

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✦ Synopsis

polC, the gene specifying the structure of the replication-specific DNA polymerase III of B. subtilis, was mapped by exploiting azp-12, a mutation conferring resistance to azopyrimidine which determines a mutant, azopyrimidine-resistant enzyme. azp-12 was located in the area of the pyrA locus and is between spcB1 and recA1. azp-12 was linked by transformation to four other mutations which influence the in vitro behaviour of DNA polymerase III--polC25, polC26, mut-1(ts), and DNAF133; the close linkage of these five mutations strongly suggests that they are alleles of the same gene.

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