✦ LIBER ✦

Bacillus subtilis dnaF: A mutation of the gene specifying the structure of DNA polymerase III

✍ Scribed by Vrooman, Mary Jane ;Barnes, Marjorie H. ;Brown, Neal C.

Publisher: Springer
Year: 1978
Tongue: English
Weight: 495 KB
Volume: 164
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00333165

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✦ Synopsis

The characteristics of Bacillus subtilis dnaF, a mutation specifying a temperature sensitive phenotype, were examined to determine its relationship to polC, the gene specifying the structure of DNA polymerase III (pol III). Exposure of growing cells bearing dnaF to non-permissive temperature inhibited replicative DNA synthesis and specifically depressed the expression of pol III activity in crude extracts. Highly purified pol III derived from cells bearing dnaF was temperature.sensitive in its polymerase activity, indicating that dnaF is a specific, polC mutation which specifies a structurally altered enzyme.

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