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Molecular cloning of Bacillus subtilis genes involved in DNA metabolism

✍ Scribed by Perego, Marta ;Ferrari, Eugenio ;Bassi, Maria Teresa ;Galizzi, Alessandro ;Mazza, Piergiorgio

Publisher
Springer
Year
1987
Tongue
English
Weight
703 KB
Volume
209
Category
Article
ISSN
0026-8925

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✦ Synopsis

Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A lambda recombinant clone carrying a chromosomal fragment able to transform dnaC mutants was obtained from a lambda Charon 4A library. A restriction map of the cloned DNA fragments was constructed. The 11.3 kb cloned DNA fragment of plasmid pMP60-13 containing the wild-type sequence of dnaG5 was shown to transform a recF33 mutant as well.

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