Alkaloids purified from plants provide many pharmacologically active compounds, including leading chemotherapy drugs. As is generally true of secondary metabolites, overall productivity is low, making commercial production expensive. Alternative production methods remain impractical, leaving the pla
Manipulating Gene Expression for the Metabolic Engineering of Plants
β Scribed by Philip A. Lessard; Harikrishna Kulaveerasingam; Gregory M. York; Amie Strong; Anthony J. Sinskey
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 134 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1096-7176
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β¦ Synopsis
Introducing and expressing foreign genes in plants present many technical challenges that are not encountered with microbial systems. This review addresses the variety of issues that must be considered and the variety of options that are available, in terms of choosing transformation systems and designing recombinant transgenes to ensure appropriate expression in plant cells. Tissue specificity and proper developmental regulation, as well as proper subcellular localization of products, must be dealt with for successful metabolic engineering in plants.
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## Abstract ## Background Optimization of gene therapy protocols requires accurate and nonβinvasive quantification of vector delivery and gene expression. To facilitate nonβinvasive imaging of gene expression, we have genetically engineered βartificial receptorsβ, i.e. membrane proteins that bind