Baculovirus expression vectors are widely used for expressing heterologous proteins in cultured insect cells. Recent advances include further development of the system for production of multi-subunit protein complexes, co-expression of protein-modifying enzymes to improve heterologous protein produc
Mammalian recombinant proteins: Vectors and expression systems
โ Scribed by Geoff T. Yarranton
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 791 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0958-1669
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## Abstract We have developed a fully contained system for expressing recombinant proteins that is based on clonal root cultures and episomal expression vectors. Clonal root lines expressing green fluorescent protein (GFP) or human growth hormone were generated from __Nicotiana benthamiana__ leaves
Fed-batch culture can offer significant improvement in recombinant protein production compared to batch culture in the baculovirus expression vector system (BEVS), as shown by Nguyen et al. (1993) and Bedard et al. (1994) among others. However, a thorough analysis of fed-batch culture to determine i