## Abstract In the presence of malate or citrate sporangiospores of __C. elegans__ were able to hydroxylate cortexolone with a rate twofold exceeding that of the control, water suspended spores. Analysis of the intracellular nicotinamide coenzyme pools revealed an increased NADPH: (NADP^+^ + NADPH)
Maintenance of steroid 11-hydroxylation activity in immobilized Cunninghamella elegans protoplasts
✍ Scribed by J. Dlugon´ski; K. Paraszkiewicz; L. Sedlaczek
- Book ID
- 110372400
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 215 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1573-0972
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Cytochrome P-450 was shown to be involved in 1 la-, and lib-hydroxylation of Substance S in intact C. elegans protoplasts. The steroid transformation was inhibited by carbon monoxide, the inhibitory effect being dependent on CO concentration. The function of cyt P-450 in intact protoplasts was confi
Spores harvested from steroid-free media (non-induced during sporogenesis), and used for cortexolone hydroxylation in the presence of cycloheximide to prevent enzyme synthesis during transformation produced cortexolone derivatives a t a low rate: 4.85 f 2.37 mg products per g dry weight per 6 hours.