Factors regulating the steroid 11-hydroxylation by non-germinating spores of Cunninghamella elegans (LENDNER)

✍ Scribed by Dr. A. Jaworski; L. Sedlaczek; D. Wilmańska; A. Sasiak; A. Strycharska

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 742 KB
• Volume: 22
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19820220506

✦ Synopsis

Abstract

In the presence of malate or citrate sporangiospores of C. elegans were able to hydroxylate cortexolone with a rate twofold exceeding that of the control, water suspended spores. Analysis of the intracellular nicotinamide coenzyme pools revealed an increased NADPH: (NADP^+^ + NADPH) ratio, indicating more effective NADPH‐generating systems in malate‐ or citrate‐stimulating spores.

Swollen spores remaining in the pregermination state, retained higher cortexolone‐hydroxylating activity in the absence of malate and citrate. In these spores degradation of endogenous alanine and glutamic acid was observed. Possible NADPH‐generating systems in C. elegans sporangiospores were discussed.