Inducible nature of the steroid 11-hydroxylases in spores of Cunninghamella elegans (Lendner)
✍ Scribed by A. Jaworski; Prof. Dr. L. Sedlaczek; J. Dlugoński; Ewa Zajaczkowska
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 324 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
✦ Synopsis
Spores harvested from steroid-free media (non-induced during sporogenesis), and used for cortexolone hydroxylation in the presence of cycloheximide to prevent enzyme synthesis during transformation produced cortexolone derivatives a t a low rate: 4.85 f 2.37 mg products per g dry weight per 6 hours.
Spores induced during sporogenesis and transformation formed the hydroxyIation products a t the rate 40.25 & 6.25 mg/g d.wt.16 h, whereas spores induced only once -on the stage of sporogenesis or during transformationproduced them a t the rate 16.28 f 4.80 -18.85 f 4.48 mg/g. 6 h-The effectiveness of the hydroxylase induction during steroid transformation could be increased if spores pretreated with diluted KOH were used. This agent wits shown to loosen the rigid structure of the spore envelopes, and to increase their permeability for the enzyme inducer. The similar could be shown with spores preincubated in a growth medium for 1-3 hours. Transformation rates of 51.34 mg/g. 6 h and 68.82 mg/g. 6 h were attained.